Restoration of membrane excitability by microinjection of cytoplasmic wild-type RNA into Paramecium tetraurelia pawn C mutants
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چکیده
منابع مشابه
Restoration of membrane excitability in a behavioral mutant of Paramecium caudatum during conjugation and by microinjection of wild- type cytoplasm
When cells of the behavioral mutant cnrC of Paramecium caudatum were mated with the wild type, phenotype change from CNR (no backward swinning) to wild type in the cnrC mate occurred immediately after the formation of tight pairs. No change of phenotype occurred when cells of cnrA or cnrB were mated with wild type. Phenotypic change from CNR to wild type in cells of cnrC was also induced by mic...
متن کاملMembrane currents of pawn mutants of the pwA group in Paramecium tetraurelia.
Membrane currents were recorded from the wild type and two pawn mutants of the pwA complementation group in Paramecium tetraurelia under a voltage clamp. Most currents are not changed by the mutations. Transient inward currents of a leaky mutant, pwA132, upon step depolarizations are less than those in the wild type. The inward transient is completely lacking in a non-leaky mutant, pwA500. The ...
متن کاملCharacterization of the cilia and ciliary membrane proteins of wild- type Paramecium tetraurelia and a pawn mutant
Cilia and ciliary membranes were isolated from axenically grown, wild-type Paramecium tetraurelia strain 51s and from the extreme pawn mutant strain, d495, derived from this parental strain. Over 60 protein bands having molecular weights of 15 to greater than 300 kdaltons were detected by Coomassie Blue staining of whole cilia proteins separated by one-dimensional SDS polyacrylamide gel electro...
متن کاملCalcium channel stability measured by gradual loss of excitability in pawn mutants of Paramecium aurelia.
Mutants of Paramecium aurelia that are unable to reverse swimming direction are called pawns. They lack the inward ionic (calcium) current required for the upstroke of the electrically excitable membrane response. By following the progressive loss of reversal response and excitability in cells that are suddenly changed from a heterozygous (wild-type) state to a homozygous mutant state, an estim...
متن کاملMicroinjection of cytoplasm as a test of complementation in Paramecium
Mutants in Paramecium tetraurelia, unable to generate action potentials, have been isolated as cells which show no backward swimming in response to ionic stimulation. These "pawn" mutants belong to at least three complementation groups designated pwA, pwB, and pwC. We have found that microinjection of cytoplasm from a wild-type donor into a pawn recipient of any of the three complementation gro...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 1982
ISSN: 0378-1097
DOI: 10.1016/0378-1097(82)90110-0